Genetic diversity of pectobacterium carotovorum subsp. This study aimed at isolating lytic bacteriophages and evaluating methods for and the efficacy of applying phages to control potato soft rot caused by pectobacterium carotovorum. In wet weather in the field, it can cause damage to the stem and. First report of blackleg and soft rot of potato caused by pectobacterium carotovorum subsp. This article is published with open access at abstract pectobacterium carotovorumsubsp. Characterization of genes required for the pathogenicity. Biocontrol efficiency of medicinal plants against pectobacterium carotovorum, ralstonia solanacearum and escherichia coli the open conference proceedings journal, 2012, 3. Complete genome sequence of the necrotrophic plant.
Pectobacterium carotovorum subpolar hyperflagellation. To identify pathogenicityrelated factors, chinese cabbage was inoculated with 5314 transposon mutants of p. Pectobacterium carotovorum and pectobacterium atrosepticum are dreadful causal agents of potato soft rot. Characterisation of pectobacterium wasabiae and pectobacterium carotovorum subsp. Pectobacterium is a bacterial genus of the family pectobacteriaceae. Here, we report the genome sequence of pectobacterium carotovorum strain scc1, a finnish soft rot model strain isolated from a diseased potato tuber in the early 1980s. Composite samples of six different tuber sections peel, stolon end, and peeled potato tissue 0. In 2010, a disease affecting lettuce lactuca sativa var. In temperate climates, the role of pectobacterium carotovorum subsp. Symptoms of fruit and pedicel decay were consistent with symptoms caused by pectobacterium carotovorum subsp. Management of pectobacterium carotovorum infections in. Pdf occurrence of pectobacterium carotovorum strains.
Panda p, vanga br, lu a, fiers m, fineran pc, butler r, armstrong k, ronson cw and pitman ar 2016 pectobacterium atrosepticum and pectobacterium carotovorum harbor distinct, independently acquired integrative and conjugative elements encoding coronafacic acid that enhance virulence on potato stems. Comparative genomic analysis of pectobacterium carotovorum. Detailed studies were conducted on the distribution of pectobacterium carotovorum subsp. Soft rot is an economically significant disease in potato and one of the major threats to sustainable potato production.
First report of soft rot by pectobacterium carotovorum subsp. General information about pectobacterium carotovorum erwica. Previously, we reported that the particular strain pcc21, producing only one highmolecularweight bacteriocin, carried a new antibacterial activity against the indicator strain pcc3. Samuel markell, extension plant pathologist, north dakota state university. In field experiments in the netherlands, the virulence of this pathogen was compared with strains of other dickeya and pectobacterium species. A novel versatile glycoside hydrolase cel5m was identified from pectobacterium carotovorum and used in ramie degumming. Bacteria of the genus pectobacterium are economically important plant pathogens that cause soft rot disease on a wide variety of plant species. Plants with leaves showing chlorotic spots and light necrotic lesions and extensive watersoaked areas at the crown. Carotovorum as causal agents of potato soft rot in lebanon. Erwinia herbicola, which is an epiphytic species and erwinia stewartii that causes stewarts wilt disease.
This disease occurred in tulip fields grown for seed bulbs of various varieties in 2002. During summer 2011 in south korea, severe fruit rot of paprika was observed, causing severe economic losses in paprika production. Strains in clade i but not clade ii were phenotypically consistent with the original description of p. In this study, we conducted the systematic identification of circrnas from time series samples of potato cultivars valor susceptible and bp1 disease tolerant infected by pcb. Bob harveson, sam markell, tom gulya and charlie block. Pectobacterium carotovorum is a gramnegative bacterium that, together with other soft rot enterobacteriaceae causes soft rot disease in vegetables, fruits, and ornamental plants through the action of exoproteins including plant cell walldegrading enzymes pcwdes. Pectobacterium wasabiae is a plant pathogenic bacterium that was first reported to cause disease on wasabi plants. Bacteriophages isolated in china for the control of. Molecular detection of the causative agent of the potato soft. To characterize the interaction between human pathogens and phytobacterial pathogens in the phyllosphere, we examined the interactions between pectobacterium carotovorum subsp. The plant pathogenic bacterial genus pectobacteirum consists of heterogeneous strains. Pectobacterium atrosepticum and pectobacterium carotovorum. In different western and central european countries plants are frequently found with blackleg from which.
Management of pectobacterium carotovorum infections in potatoes solanum tuberosum l. Pectobacterium atrosepticum is a species of bacterium. A collection of 42 strains belonging to the five subspecies of pectobacterium carotovorum subspecies atrosepticum, betavasculorum, carotovorum, odoriferum and wasabiae and 11 reference and type strains of biovars of pectobacterium chrysanthemi, pectobacterium cacticidum and brenneria paradisiaca were studied by dnadna hybridization, numerical taxonomy of 120 phenotypic. Pectobacterium carotovorum simple english wikipedia, the. Characterization of a versatile glycoside hydrolase cel5m. Blackleg disease has resulted in significant economic losses and continues to devastate the potato industry 2, 3. These permits may be required for shipping this product. Pectobacterium carotovorum subpolar hyperflagellation shinichi aizawa, in the flagellar world, 2014 pectobacterium formerly erwinia carotovorum is a gramnegative plantspecific pathogen, causing soft rot disease of various plant hosts and blackleg in potato by degradation of the plant cell wall. Its type strain is cfbp 3304 t lmg 8404 t ncppb 3701 t icmp 9121 t. Taxonomic relatedness between pectobacterium carotovorum subsp. The etiology of a new soft rot disease of tulips, causing leaf spots, leaf blight, neck and bulb rot in konya, turkey, was investigated. Batch, fed batch production and characterization of.
Taxonomic relatedness between pectobacterium carotovorum. It is well established that the pectinolytic bacteria pectobacterium atrosepticum pca and dickeya spp. Department of agricultural sciences, university of helsinki, helsinki, finland. Its type strain is cfbp 1526 t lmg 2386 t ncppb 549 t icmp 1526 t. Pdf biocontrol of pectobacterium carotovorum subsp. Pectobacterium carotovorum causes the bacterial soft rot bsr diseases in many crops and ornamentals, ranging from carrots and potatoes to leafy greens and irises. Erwinia, pectobacterium, and brenneria species include pectobacterium carotovorum references. A closely related species, yet to be formally named, also causes disease on potato. Pectobacterium carotovorum subpolar hyperflagellation shinichi aizawa, in the flagellar world, 2014 pectobacterium formerly erwinia carotovorum is a gramnegative plantspecific pathogen, causing soft rot disease of various plant hosts and blackleg in. Complete genome sequence of the necrotrophic plantpathogenic. H7 with regard to bacterial populations, soft rot progression, and changes in local ph. Primarily, this pathogen causes postharvest issues for fruits and vegetables 7. Control of potato soft rot caused by pectobacterium. Usda aphis ppq permit 526, plant pathogens permit must be obtained and a copy of the permit must be sent to atcc in advance of shipment.
Gene expression data identified pectobacterium carotovorum subsp. An easy, simple inexpensive test for the specific detection of pectobacterium carotovorum subsp. This causes the cells to separate, a disease plant. Characterization of pectobacterium carotovorum subsp. Genome sequence of the model plant pathogen pectobacterium. Cloning and expression of pectobacterium carotovorum endo. Biological control using actinobacteria could be an interesting approach to manage this disease. Analysis of these genome sequences will help to pinpoint differences in virulence among p. In western europe, pectobacterium carotovorum subsp. The third group, herbicola consists of two main species. Characterization of a new bacteriocin, carocin d, from. Characterisation of pectobacterium wasabiae causing blackleg and soft rot diseases in south africa lucy n moleleki, edward n onkendi, aobakwe mongae and gugulethu c kubheka moleleki ln department of microbiology and plant pathology, university of pretoria, south africa forestry agriculture and biotechnology institute email.
Genomeinformed diagnostics for specific and rapid detection. Pectobacterium brasiliense has been reported as one of the most aggressive pectobacterium species known to date. It produces pectolytic enzymes that hydrolyze pectin between individual plant cells. Little information about the roles of circular rnas circrnas during potato pectobacterium carotovorum subsp.
Research was conducted to identify 91 pectobacterium spp. It is a plant pathogen and opportunistic human pathogen, causative agent of soft rot and blackleg potato p. View enhanced pdf access article on wiley online library html view download pdf for offline viewing. Pectobacterium carotovorum syn erwinia carotovora is a rodshaped bacterium originally isolated from carrots. Pdf characterization of pectobacterium carotovorum subsp. Two different bacteriocins, carotovoricin and carocin s1, had been found in pectobacterium carotovorum subsp.
Soft rot disease severity is affected by potato physiology. However, other virulence factors and the mechanisms of genetic adaptation still need to be studied in detail. Park th, choi bs, choi ay, choi iy, heu s, park bs. Of fifty bacterial isolates with white grey and convex colonies on nutrient agar obtained from symptomatic watermelon, ten. The structure of anthranilate phosphoribosyltransferase from the enterobacterium pectobacterium carotovorum has been solved at 2. Pdf an easy, simple inexpensive test for the specific. The aim of this study was characterized pectobacterium carotovorum subsp. Cloning and expression of pectobacterium carotovorum endopolygalacturonase gene in pichia pastoris for production of oligogalacturonates. Production of plant cell wall degrading enzymes is usually critical for pectobacterium infection. Groves, department of entomology, university of wisconsinmadison. Microorganisms free fulltext the gene encoding nad. A collection of 42 strains belonging to the five subspecies of pectobacterium carotovorum subspecies atrosepticum, betavasculorum, carotovorum, odoriferum and wasabiae and 11 reference and type strains of biovars of pectobacterium chrysanthemi, pectobacterium cacticidum and brenneria paradisiaca were studied by dnadna hybridization, numerical taxonomy of 120 phenotypic characteristics. Ivanovic m, gasic k, gavrilovic v, obradovic a, 2009. Pectobacterium aroidearum and pectobacterium carotovorum.
Pdf bacterial leaf and peduncle soft rot caused by. Pectobacterium carotovorum an overview sciencedirect topics. In 20 and 2014, seed potato tubers were vacuum infiltrated with high densities of bacteria 10 6 cfu ml. Genome sequence of pectobacterium carotovorum subsp.
Nov 22, 2019 ivanovic m, gasic k, gavrilovic v, obradovic a, 2009. Here, we report the draft genome sequences of three weakly virulent p. Pectobacterium species are necrotrophic bacterial pathogens that cause soft rot diseases in potatoes and several other crops worldwide. Pectobacterium carotovorum, pseudomonas putida, proteus vulgaris and proteus mirabilis strains pretreated with chloroform or polymyxin b to remove the external membrane. Pectobacterium carotovorum is a bacterium of the family pectobacteriaceae. Eleven bacteriophages isolated from soil and water samples collected in wuhan, china. Pectobacterium formerly erwinia carotovorum is a gramnegative plantspecific pathogen, causing soft rot disease of various plant hosts and blackleg in potato by degradation of the plant cell. Elevation of three subspecies of pectobacterium carotovorum. Occurrence of pectobacterium carotovorum strains isolated from potato soft rot in morocco. Actually, there are no efficient bactericides used to protect potato against pectobacterium spp. Nagina rafique, a romana tabassum, b muhammad siddique awan, c william orts, d and dominic w. Pectobacterium carotovorum an overview sciencedirect. Salmonella enterica suppresses pectobacterium carotovorum. Pectobacterium aroidearum and pectobacterium carotovorum subsp.
1362 1102 1248 781 154 1151 595 714 178 543 280 998 1057 872 944 1515 488 79 199 1380 33 480 1192 24 344 1483 353 1514 172 640 497 175 306 1037 14 1414 260 42 13 662 887 429 1280 502 872 454